GIST stained with Anti-DOG1_
DOG1 is a calcium-dependent chloride channel protein that is encoded by a gene called TMEM16A (TMEM16 FLJ0261, ANO1, ORAOV2, and AOS2) located on chromosome 11q13. DOG1 has many significant functions such as regulation of the cholinergic activity of gastrointestinal smooth muscle and regulation of both the survival and proliferation of cells. DOG1 is detected in gastrointestinal Cajal cells, acinic cells in salivary glands (apical membraneous staining, particularly in serous cells), pancreatic centroacinar cells, liver cells, and epithelium of biliary tract, breast, stomach, and prostate. More than 90% of all gastrointestinal stromal tumors (GISTs) are DOG1 positive, irrespective of kit mutation and CD117 positivity. The staining pattern varies from cytoplasmic to membranous, with usually strong, diffuse intensity. DOG1 is an important marker in the identification of GIST together with CD117, slightly more sensitive (particularly in gastric GIST without c-kit mutation) and also more specific than CD117. DOG1 is also useful in the classification of salivary carcinomas, and pancreatic and renal tumors
DOG1 is a calcium-dependent chloride channel protein that is encoded by a gene called TMEM16A (TMEM16 FLJ0261, ANO1, ORAOV2, and AOS2) located on chromosome 11q13. DOG1 has many significant functions such as regulation of the cholinergic activity of gastrointestinal smooth muscle and regulation of both the survival and proliferation of cells. DOG1 is detected in gastrointestinal Cajal cells, acinic cells in salivary glands (apical membraneous staining, particularly in serous cells), pancreatic centroacinar cells, liver cells, and epithelium of biliary tract, breast, stomach, and prostate. More than 90% of all gastrointestinal stromal tumors (GISTs) are DOG1 positive, irrespective of kit mutation and CD117 positivity. The staining pattern varies from cytoplasmic to membranous, with usually strong, diffuse intensity. DOG1 is an important marker in the identification of GIST together with CD117, slightly more sensitive (particularly in gastric GIST without c-kit mutation) and also more specific than CD117. DOG1 is also useful in the classification of salivary carcinomas, and pancreatic and renal tumors
