Special Stains

Special Stains

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  • Used to identify acid-fast organisms
  • Other cells/tissue components are counter stained with light green
  • Comes in a convenient kit form

AFB
  • Stains the acid mucosubstances and acetic mucins
  • Strongly acidic mucosubstances are stained blue
  • Nuclei are stained pink to red
  • Cytoplasm is stained pale pink
  • Comes in a convenient kit form

Alcian Blue
  • Used for the differential staining of elastic fibers
  • Gold standard technique for the detection of amyloidosis
  • Comes in a convenient kit form

Congo Red
  • Used in the histologic visualization of fungi, basement membrane
  • Used in the indentification of some opportunistic organisms such as Pneumocystis carinii
  • Comes in a convenient kit form

G.M.S
  • Used to study muscular dystrophy, infarct, cirrhosis or glomerular fibrosis
  • Used to detect and analyze tumors on hepatic and kidney biopsies
  • Three dyes are employed to selectively stain muscle, collagen fibers, fibrin, and erythrocytes
  • Comes in a convenient kit form

Massons Trichome
  • Used in the histological visualisation of acid mucopolysacharides in tissue sections
  • Used for staining the capsule of the fungus Cryptococcus neoformans
  • Comes in a convenient kit form

Mucicarmine
  • Used to demonstrate neutral fats in liver tissue
  • Used to assess the presence and extent of neutral fat
  • Comes in a convenient kit form

Oil Red O
  • Polychrome staining method which depends on degree of cellular maturity and cellular metabolic activity
  • Designed to differentiate variety of cells in vaginal smear to detect vaginal, uterine and cervical cancers
  • Comes in a convenient kit form

PAP (Papanicolaou) Stain- Harris Hematoxylin
  • Polychrome staining method which depends on degree of cellular maturity and cellular metabolic activity
  • Designed to differentiate variety of cells in vaginal smear to detect vaginal, uterine and cervical cancers
  • Comes in a convenient kit form

PAP (Papanicolaou) Stain- Modified Mayer’s Hematoxylin
  • Used to detect the polysaccharides or molecules with high percentage of carbohydrate content such as glycogen and mucosubstances such as glycoproteins, glycolipids and mucins in tissues
  • Intended to demonstrate lymphocytes in tissues
  • Comes in a convenient kit form

PAS
  • Used in combination with diastase enzyme to differentiate glycogen from PAS-positive elements in tissue samples
  • Comes in a convenient kit form

PAS - Diastase
  • Used to identify acidic and neutral mucins
  • It separates the acidic and neutral mucins by color and can be used to distinguish all mucins in tissue sections
  • Comes in a convenient kit form

PAS Alcian Blue
  • Used special stain to demonstrate reticulin fibers
  • Comes in a convenient kit form

Reticulin
  • Tissue is stained with hematoxylin-ferric chloride and iodine
  • Comes in a kit form

VVG-Verhoeff Vangieson
  • Best staining technique to detect spirochaetes as well as small bacilli
  • Comes in a convenient kit form

Warthin Starry
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