Tris EDTA buffer is a high pH, 9.0 alkaline retrieval solution. The effects of pH on different formaldehyde-fixed epitopes are due to their different constituent amino acids linked to formaldehyde being more easily broken in alkaline medium. It is mostly used for nuclear markers and tends to pick some background.
Tris EDTA buffer is a high pH, 9.0 alkaline retrieval solution. The effects of pH on different formaldehyde-fixed epitopes are due to their different constituent amino acids linked to formaldehyde being more easily broken in alkaline medium. It is mostly used for nuclear markers and tends to pick some background.