Chromogenic Substrates
Visualization is an important tool in Immunohistochemistry process. The binding of primary and secondary antibodies in the process is illuminated by the enzymatic reaction of Horseradish peroxidase and chromogenic substrates such as DAB(3,3’-Diaminobenzidine) and AEC (3-Amino-9-EthylCarbazole). The biochemical reaction converts the substrate into a pigment reaction product, which produces a color such as Brown for DAB and Red for AEC. PathnSitu offers high sensitive StunnDAB Chromogen and buffer for Immunohistochemical application. It develops faster color and has longer stability of working solution up to 5 days when stored at 2-8°C. The chocolate/ golden brown color develops during reaction is very distinguished and appealing under microscopic examination.
Visualization is an important tool in Immunohistochemistry process. The binding of primary and secondary antibodies in the process is illuminated by the enzymatic reaction of Horseradish peroxidase and chromogenic substrates such as DAB(3,3’-Diaminobenzidine) and AEC (3-Amino-9-EthylCarbazole). The biochemical reaction converts the substrate into a pigment reaction product, which produces a color such as Brown for DAB and Red for AEC. PathnSitu offers high sensitive StunnDAB Chromogen and buffer for Immunohistochemical application. It develops faster color and has longer stability of working solution up to 5 days when stored at 2-8°C. The chocolate/ golden brown color develops during reaction is very distinguished and appealing under microscopic examination.
